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M94A0638.TXT
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1994-10-21
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Document 0638
DOCN M94A0638
TI Factors involved in susceptibility of macrophages but not fresh
monocytes to HIV infection.
DT 9412
AU Maerz A; Sonza S; Crowe S; Macfarlane Burnet Centre for Medical
Research, Fairfield,; Victoria.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:65 (abstract no. TB8).
Unique Identifier : AIDSLINE ASHM5/94349017
AB OBJECTIVES: To determine the time post isolation at which
monocyte/macrophages show increased susceptibility to infection with
HIV, and identify the stage of viral replication which is blocked in
cells which are less susceptible. METHODS: Monocytes were isolated from
HIV seronegative buffy coats by density gradient centrifugation and
plastic adherence. Cells were infected with HIV-1 (BaL) on Day 0, 1, 2
and 7 post isolation. HIV infection was quantitated by PCR using primer
pairs which detect all initiated, partially completed and almost full
length cDNA transcripts, intracellular p24 quantification by MAb
staining and flow cytometric analysis or p24 antigen in supernatant by
EIA. RESULTS: Freshly isolated monocytes are generally not susceptible
to infection with HIV. At least 24 hours in culture is required before
any sign of viral replication can be detected. CONCLUSION: There is a
block to HIV infection in freshly isolated monocytes which is overcome
when cells have been cultured for 24 hours. Further studies are ongoing
to compare internalization of virus, linearization of DNA, translocation
of DNA from cytoplasm to nucleus and NFkB activity in the two
populations.
DE Cells, Cultured Human HIV/*PATHOGENICITY HIV Seronegativity
Macrophages/*MICROBIOLOGY Monocytes/*MICROBIOLOGY Virulence Virus
Replication/*PHYSIOLOGY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).